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MonoRab™ GS Linker Antibody iFluor 647, mAb, Rabbi
產(chǎn)品簡介：

MonoRab™ GS Linker Antibody [iFluor 647], mAb, Rabbit：This product recognizes (GnS)m linker(n≥2, m≥2) in proteins。

產(chǎn)品型號：A02311

更新時間：2025-05-19

廠商性質(zhì)：代理商

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產(chǎn)品介紹

This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker and (G4S)3 linker, etc.


產(chǎn)品概述

SpecificityThis product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker, (G4S)2 linker and (G4S)3 linker, etc.
Host SpeciesRabbit
ImmunogenA synthetic peptide (GGGGSGGGGSGGGGS) coupled to BSA
ConjugateiFluor 647










應(yīng)用指導

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.


ApplicationRecommended Usage
Flow Cytometry1:50






產(chǎn)品屬性

FormLiquid
Storage BufferSupplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.03% ProClin300 and 50% glycerol.
Storage InstructionsStore at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.
PurificationProtein A affinity column
IsotypeRabbit IgG, κ
ClonalityMonoclonal
Clone ID20H7
NoteGenScript can customize this product per customer's request including product size, buffer components, etc.



















圖例


技術(shù)背景

Target BackgroundA linker is an amino acid chain that connects two fused proteins, allowing for the appropriate separation of protein functional domains or the maintenance of necessary inter-domain interactions. The most commonly used flexible linker is the poly-Glycine-Serine (GS) linker. A very important application of the flexible GS linker is connecting the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs). Due to its flexible structure and appropriate linker sequence length, the (GGGGS)3 linker has been shown to allow for the correct orientation of the VH and VL domains, and it does not interfere with the folding of the protein domains in scFv construction[1,2].
SynonymsGS Linker;Glycine-serine linker; (G4S)3 Linker; poly-Glycine-Serine; (GGGGS)3;
Reference[1] Huston,J,S,et al.Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.[J].Proceedings of the National Academy of Sciences, 1988, 85(16):5879-5883.
[2] Chen X , Zaro J ,Shen, Wei‐Chiang.Fusion Protein Linkers: Effects on Production, Bioactivity, and Pharmacokinetics[M].John Wiley & Sons, Inc. 2013.


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